The Molecular Biology and Proteomics Core Facilities (MBPCF)

Provide molecular biology and proteomics equipment and services that are of critical relevance in biomedical science. MBPCF provide research facilities and services of sample analysis (from sample preparation up to data analysis) and consultation. The core facilities have professional equipment and advanced technology platforms, which include oligonucleotide synthesis, protein characterization system, ultra centrifuge (up to 65000 rpm). The cluster organize training program in molecular biology and proteomics analysis for individual and groups regularly. They also arrange research consultation involving expertise in genomics and proteomics study.

Ongoing Research

  • Genome Editing CRISPR/Cas9 Services in IMERI Using MnSOD Gene Knock Out as a Model.

Publication

  • Retnoningrum, D.S., Arumsari, S., Selenia, E. D., Tandra, Y. S., Ismaya, W.T. 2018. Leu169Trp substitution in MnSOD from Staphylococcus equoroum created an active new form of similar resistance to the UVC irradiation (Enzyme and Microbial Technology 118. (2018), 13–19.
  • Sen, L.T., Septyani, J., Halim, J., Santoso, B., Ramadhan, M. B., Syahrani, R. A., Wanandi, S. I. 2018. The association of HIF-2α expression with stemness and survival genes in human breast cancer stem cells (CD24−/CD44+) exposed to hypoxia. Journal of Physics: 1073 (2018) 032066. doi: 10.1088/1742-6596/1073/3/032066.
  • Humonobe, A. I., Syahrani, R. A., Wanandi, S. I. 2018. Effect of hypoxia on ALDH1A1 expression in MCF-7 human breast cancer cells and its correlation with Oct-4 pluripotency gene expression. Journal of Physics: Conference Series. 1073 (2018) 032062. doi :10.1088/1742-6596/1073/3/032062.
  • Dewi, S., Syahrani, R. A., Sadikin, M., Wanandi, S. I. 2018. The Sensitivity of Human Breast Cancer Stem Cells (ALDH+) Against Doxorubicin Treatment is Associated with PCNA and BIRC5 Gene Expressions. (Hiroshima Journal Medical of Sciences 67. (2018), 84–90.

Collaboration

  • USAID

Organization